In this thesis we will describe in detail the beginnings of road transport in the UK and the Republic of Croatia in terms of safety regulations. In addition to the history and development of safety regulations, we will mostly deal with regulations, rules and part of the work relating to the road safety in these two states. In this thesis we will describe the fundamental differences between the roads, traffic signs and traffic lights, the obligations for vehicles and drivers in the UK and Croatia. We processed the statistical number of accidents and deaths in road traffic, which are indicators on the state of safety regulations. At the end of the thesis, we will address future plans and the development of safety regulations and how it could be improved from the aspect between the UK and Croatia.